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Development and validation of an automated extraction method (accelerated solvent extraction) and a reverse-phase
Andreas M Abend1, Le Chung, David G McCollum
1Merck & Co, Inc, Merck Research Laboratories, Sumneytown Pike, Mail Stop WP 78-210, West Point, PA 9486, USA. andreas_abend@merck.com
Journal of Pharmaceutical and Biomedical Analysis
|April 2, 2003
Abstract:
A new method for monitoring ivermectin content in HEARTGARD CHEWABLES has been developed and validated. The method consists of the automated extraction of ivermectin from the meat-based formulation under conditions of elevated temperature and pressure (accelerated solvent extraction, ASE, and determination of the active by reverse-phase high performance liquid chromatography (HPLC). The method resolves both active species of ivermectin (components H(2)B(1a) and H(2)B(1b)) from the formulation matrix.