A modified ortho-toluidine reagent for glucose analysis

Area of Science:

• Clinical Chemistry
• Analytical Chemistry

Background:

• Accurate glucose determination is crucial for diagnosing and managing diabetes.
• Traditional ortho-toluidine methods for glucose measurement have limitations.

Purpose of the Study:

• To develop and validate a modified ortho-toluidine reagent for glucose determination.
• To improve the sensitivity and efficiency of glucose assays.

Main Methods:

• A modified ortho-toluidine reagent was prepared with significantly reduced glacial acetic acid content.
• The micromethod's performance was evaluated against the original ortho-toluidine method.
• The method's adherence to Beer's Law across various concentrations was assessed.

Main Results:

• The modified reagent resulted in a two to three times higher Schiff's base color intensity compared to the original reagent.
• The proposed micromethod demonstrated good correlation with the established ortho-toluidine method.
• The reaction linearity followed Beer's Law over a broad range of glucose concentrations.

Conclusions:

• The modified ortho-toluidine micromethod provides a sensitive and reliable approach for glucose determination.
• This optimized reagent offers enhanced color intensity and reduced acid content, improving assay performance.
• The method's adherence to Beer's Law ensures accurate quantification of glucose levels.