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Updated: Aug 2, 2026

07:16
Biochemical Titration of Glycogen In vitro
Published on: November 24, 2013
A modified ortho-toluidine reagent for glucose analysis
Abstract:
A micromethod for the determination of glucose using a modified ortho-toluidine reagent is described. The proposed method correlates well with the original o-toluidine method. The reaction follows Beer's Law over a wide range of concentrations. The modified reagent contains 75 per cent less glacial acetic acid than the original ortho-toluidine reagent. The color intensity of the Schiff's base is two to three times higher than the original reagent.
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