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08:40

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Published on: November 29, 2016

Csk regulates integrin-mediated signals: involvement of differential activation of ERK and Akt

Jianguo Gu1, Shigeyuki Nada, Masato Okada

  • 1Division of Protein Chemistry, Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan. jgu@protein.osaka-u.ac.jp

Biochemical and Biophysical Research Communications
|April 3, 2003
PubMed

Insights

Csk protein regulates cell signaling pathways involved in cell adhesion and survival. Its absence affects extracellular signal-regulated kinase/mitogen-activated protein kinase and Akt activation, impacting cell proliferation and apoptosis.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Csk (C-terminal Src kinase) is a known negative regulator of Src family tyrosine kinases.
  • Integrins are cell surface receptors crucial for cell adhesion and signaling.

Purpose of the Study:

  • To investigate the role of Csk in integrin-mediated signaling pathways.
  • To understand how Csk deficiency affects cellular behaviors like proliferation and apoptosis.

Main Methods:

  • Utilized Csk-deficient fibroblasts and stable transfectants re-expressing Csk.
  • Analyzed integrin-mediated activation of ERK/MAP kinase and Akt signaling.
  • Assessed cellular responses including apoptosis and proliferation.

Main Results:

  • Csk deficiency altered integrin-mediated signaling, decreasing ERK/MAP kinase activation and increasing Akt activation.
  • Overexpression of dominant-negative Akt partially restored ERK/MAP kinase signaling.
  • Csk-deficient cells showed increased resistance to apoptosis but reduced proliferation.

Conclusions:

  • Csk plays a significant role in regulating integrin-mediated signaling pathways.
  • Csk influences cellular behaviors, including apoptosis and proliferation, through modulation of key signaling molecules.

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