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A novel sequence framework (bla(TEM-1G)) encoding the parental TEM-1 beta-lactamase

Constança Pomba-Féria1, Manuela Caniça

  • 1CIISA, Faculty of Veterinary Medicine, Universidade Técnica de Lisboa, Rua Prof. Cid dos Santos, 1300-477 Lisboa, Portugal.

FEMS Microbiology Letters
|April 3, 2003
PubMed

Insights

A novel beta-lactamase gene, bla(TEM-1G), was identified in a canine Escherichia coli strain. Phylogenetic analysis reveals close evolutionary links between canine and human beta-lactamase genes.

Area of Science:

  • Microbiology
  • Genetics
  • Molecular Biology

Background:

  • Beta-lactamase genes are crucial in bacterial antibiotic resistance.
  • Escherichia coli is a common pathogen in both human and animal infections.
  • Understanding the origin and evolution of resistance genes is vital for public health.

Purpose of the Study:

  • To characterize a novel parental bla(TEM) gene isolated from a canine uropathogenic Escherichia coli.
  • To investigate the phylogenetic relationship of this gene with other bla(TEM) genes.

Main Methods:

  • Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis.
  • Nucleotide sequencing of the bla(TEM-1G) gene.
  • Molecular phylogenetic analysis of parental bla(TEM) genes.

Main Results:

  • A novel gene, bla(TEM-1G), encoding TEM-1 beta-lactamase was identified in a canine E. coli strain.
  • The bla(TEM-1G) gene shares high sequence identity with bla(TEM-1C), differing by a single silent mutation.
  • Phylogenetic analysis grouped bla(TEM-1G) with bla(TEM-1C), suggesting it as a probable ancestor to other bla(TEM) variants.
  • The bla(TEM-1G) gene framework is similar to genes found in human E. coli strains, including those conferring inhibitor resistance.

Conclusions:

  • Parental bla(TEM) genes in E. coli from different hosts (canine and human) can be phylogenetically very close.
  • This finding has implications for understanding the transmission and evolution of antibiotic resistance genes across species.

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