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Alternative splicing modulation by a LAMMER kinase impinges on developmental and transcriptome expression.

Sigal Savaldi-Goldstein1, Dvora Aviv, Olga Davydov

  • 1Department of Plant Sciences, Weizmann Institute of Science, Rehovot, Israel.

The Plant Cell
|April 3, 2003
PubMed
Summary

The LAMMER motif kinase influences plant development by altering alternative splicing. High expression leads to developmental abnormalities and changes in gene expression related to protein turnover.

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Area of Science:

  • Molecular Biology
  • Plant Science
  • Genetics

Background:

  • Alternative splicing significantly contributes to genome complexity and cellular functions.
  • The spliceosome machinery processes nuclear RNA, with splicing factors often phosphorylated by LAMMER motif kinases.

Purpose of the Study:

  • To investigate the role of the LAMMER motif in subnuclear localization.
  • To explore the impact of LAMMER kinase on alternative splicing, plant development, and transcriptome profiles.

Main Methods:

  • Demonstration in BY-2 tobacco cells.
  • High expression of LAMMER kinase in Arabidopsis plants.
  • Transcriptome profiling and analysis of gene expression.

Main Results:

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  • The LAMMER motif is crucial for maintaining proper subnuclear localization.
  • High LAMMER kinase expression modulated alternative splicing of endogenous genes.
  • Observed abnormal plant development and a distinct transcriptome profile, with upregulated protein turnover genes.

Conclusions:

  • Phosphorylation activity by LAMMER kinase modulates alternative splicing.
  • This provides a mechanism to study the global effects of alternative splicing on plant development and gene expression plasticity.