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Updated: Aug 18, 2026

Collection of Post-mating Semen from the Female Reproductive Tract and Measurement of Semen Liquefaction in Mice
Published on: November 18, 2017
Detection of intravaginal spermatozoa after natural mating in the bitch
M. D. Whitacre1, D. J. Yates, S. D. VanCamp
1College of Veterinary Medicine, North Carolina State University, 4700 Hillsborough Street, Raleigh, NC 2760.
Abstract:
Mismating is one of the most frequently presented reproductive problems presented to veterinarians. Confirming whether or not a mismating has occurred is necessary to determine if therapy will be instituted. Detection of spermatozoa in the vagina is irrefutable evidence of copulation; however, absence of sperm in a vaginal cytology cannot rule out coitus. In order to improve detection of spermatozoa post-coitus, a prospective study was initiated utilizing natural breedings of purebred beagles.
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