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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
Published on: June 15, 2016
IFN-gamma-mediated inhibition of antigen receptor-induced B cell proliferation and CREB-1 binding activity requires
Frank Frissora1, Hui-Chen Chen, Joan Durbin
1Children's Research Institute, Wexner Institute for Pediatric Research, Columbus, OH 43205, USA.
Abstract:
We report here a role for cyclic AMP-responsive element-binding protein-1 (CREB-1) in B cell antigen receptor (BCR)-induced growth inhibition by IFN-gamma. BCR-induced proliferation is negatively regulated by IFN-gamma. Stimulation through BCR resulted in dose-dependent induction of CREB-1 binding to the consensus cyclic AMP-responsive element. Recombinant IFN-gamma inhibited the BCR-induced CREB-1 DNA binding activity and cell proliferation in B cells from signal transducer and activator of transcription-1 (STAT-1)(+/+), but not STAT-1(-/-) mice. These studies provide the first evidence for cross-talk between the STAT-1 and CREB-1 signaling pathways in IFN-gamma-mediated negative regulation of B cell activation.
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