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Optical imaging fiber-based live bacterial cell array biosensor.

Israel Biran1, David M Rissin, Eliora Z Ron

  • 1The Max Tishler Laboratory for Organic Chemistry, Department of Chemistry, Tufts University, Medford, MA 02155, USA.

Analytical Biochemistry
|April 4, 2003
PubMed
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Researchers developed a novel mercury biosensor using a single bacterial cell array on an optical imaging fiber. This sensitive platform detects mercury ions (Hg2+) by monitoring genetically engineered E. coli responses.

Area of Science:

  • Biotechnology and Biosensing
  • Microfluidics and Lab-on-a-Chip Devices
  • Environmental Monitoring

Background:

  • Development of sensitive and specific biosensors is crucial for environmental monitoring.
  • Bacterial cells offer a versatile platform for detecting specific analytes through genetic engineering.
  • Optical imaging fibers provide a high-density array format for single-cell analysis.

Purpose of the Study:

  • To fabricate a live cell array biosensor using an optical imaging fiber.
  • To develop a mercury biosensor utilizing a genetically engineered Escherichia coli strain.
  • To demonstrate the capability of detecting mercury ions at the single-cell level.

Main Methods:

  • Immobilization of genetically modified bacterial cells within microwells on an optical imaging fiber.

Related Experiment Videos

  • Engineering of Escherichia coli with a lacZ reporter gene fused to a heavy metal-responsive promoter (zntA).
  • Introduction of enhanced cyan fluorescent protein (ECFP) for cell localization.
  • Measurement of single-cell lacZ expression upon exposure to mercury (Hg2+).

    • Main Results:

    • Successful fabrication of a single bacterial cell array biosensor on an optical imaging fiber.
    • Detection of mercury ions (Hg2+) with a response observed at 100nM concentration.
    • A 1-hour incubation time was sufficient for detecting mercury exposure.
    • ECFP enabled clear identification of individual bacterial cell locations within the array.

    Conclusions:

    • The optical imaging fiber-based single bacterial cell array is a flexible and sensitive biosensor platform.
    • This platform can be adapted to monitor various reporter gene expressions.
    • The system accommodates diverse sensing bacterial strains for multiple analyte detection.