Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Solution structure of a functionally active fragment of decay-accelerating factor.

Stanislava Uhrinova1, Feng Lin, Graeme Ball

  • 1Edinburgh Protein Interaction Centre, University of Edinburgh, Edinburgh EH9 3JJ, Scotland.

Proceedings of the National Academy of Sciences of the United States of America
|April 4, 2003
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Fast and Reliable NMR-Based Fragment Scoring for Drug Discovery.

Journal of the American Chemical Society·2025
Same author

MS and NMR Analysis of Isotopically Labeled Chloramination Disinfection Byproducts: Hyperlinks and Chemical Reactions.

Analytical chemistry·2024
Same author

Quantification of whisky congeners by <sup>1</sup>H NMR spectroscopy.

Analytical science advances·2024
Same author

Clean PDI-1 SQ: Suppression of HSQC artifacts in 2D proton-detected INADEQUATE spectra by pulse sequence redesign.

Journal of magnetic resonance (San Diego, Calif. : 1997)·2024
Same author

SHARPER-DOSY: Sensitivity enhanced diffusion-ordered NMR spectroscopy.

Nature communications·2023
Same author

Enhancing <sup>19</sup>F Benchtop NMR Spectroscopy by Combining <i>para</i>-Hydrogen Hyperpolarization and Multiplet Refocusing.

ACS measurement science au·2023

The structure of decay accelerating factor (DAF) modules 2 and 3 was determined, revealing how it binds and regulates complement pathways. This finding is crucial for understanding complement system regulation and developing new therapies.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Immunology

Background:

  • The complement system is crucial for innate immunity.
  • Decay accelerating factor (DAF) regulates complement activation.
  • No prior structural data existed for mammalian decay-accelerating proteins.

Purpose of the Study:

  • Determine the solution structure of DAF modules 2 and 3 (DAF ≈ 2,3).
  • Identify the interaction sites between DAF and the classical pathway (CP) convertase.
  • Elucidate the mechanism of DAF-mediated complement regulation.

Main Methods:

  • Solution structure determination of DAF ≈ 2,3 using NMR.
  • Structure-guided mutagenesis of DAF (24 mutants).
  • Analysis of DAF-CP convertase interactions.

Related Experiment Videos

Main Results:

  • The solution structure of DAF ≈ 2,3 was determined.
  • Key contact residues on both DAF and the CP convertase were identified.
  • DAF binds to the CP convertase in a groove, engaging both concave and convex faces near the module 2-3 junction.

Conclusions:

  • DAF ≈ 2,3 is sufficient for classical pathway convertase decay acceleration.
  • The binding mode suggests a simultaneous interaction involving multiple DAF surfaces.
  • Understanding these interactions provides insights into complement regulation and potential therapeutic targets.