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Updated: Aug 11, 2026

Isolation of Human Lymphatic Endothelial Cells by Multi-parameter Fluorescence-activated Cell Sorting
Published on: May 1, 2015
It is true that, when Langerhans cells migrate from the skin to the lymph node, they are transported via lymph
Keiji Sugiura1, Mikihiro Shamoto, Nao Sakamoto
1Division of Pathological Cytology, Institute for Comprehensive Medical Science, Fujita Health University, Toyoake, Japan. ksugiura@med.nagoya-u.ac.jp
Background:
Generally, Langerhans cells deliver antigen information from the skin to the draining lymph nodes via lymph vessels.
Methods:
By immunohistopathology, we investigated the delivery route of Langerhans cells in human skin using CD1a and S-100 protein antibodies.
Results:
We noted CD1a- and S-100-positive Langerhans cells in the lymph vessels of the dermis. These were shaped like dendritic cells and presented with some lymphocytes, melanophages, melanin granules and lymph in the same vessels.
Conclusion:
These observations support the concept that Langerhans cells deliver antigen peptides to regional lymph nodes via afferent lymph vessels.
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