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Updated: Sep 26, 2026
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Published on: February 6, 2018
[Molecular cloning of TSARG3 gene related to apoptosis in human spermatogenic cells]
Gang Liu1, Guangxiu Lu, Junjiang Fu
1Human Reproductive Engineering Institute, Xiangya Medical School, Central South University, Changsha, Hunan, 410078 P. R. China. lgxdiredtor@sina.com
Objective:
To clone a novel gene which is related to human testis spermatogenesis apoptosis.
Methods:
To rapidly attain human novel gene full-length cDNA sequence from a human testis cDNA library,the gene-specific primers and the vector-specific primers were designed for nested polymerase chain reaction. Sequencing was performed and the result was analysed.
Results:
The present authors discovered the TSARG3 gene(GenBank accession number AF419291) from a human testis cDNA library, using a cDNA fragment (GenBank accession number BE644537) as an electronic probe, which was significantly changed in cryptorchidism and represented a novel gene. Furthermore, a mouse homologue of this gene was identified (GenBank accession number AF419292) by using the same method.
Conclusion:
A novel gene named TSARG3 was cloned. It is considered that the function of the new gene is related to human testis spermatogenesis apoptosis.
