[Cloning and mapping analysis of cDNA fragment OPB7-1 gene in human lung adenocarcinoma]
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Published on: January 19, 2019
1Laboratory of Medical Genetics, Harbin Medical University, Harbin, Helongjiang, 150086 P. R. China. lipu@ems.hrbmu.edu.cn
Objective:
To search the candidate gene in the development and metastasis of lung adenocarcinoma and shed light on the possible molecular mechanism of the development of lung carcinoma.
Methods:
Using methods of cell culture, reverse transcription-PCR, RH gene mapping and RNA in situ hybridization.
Results:
The cDNA fragment named OPB7-1 was mapped at 1p31-1p34 by RH gene mapping method. The fragment sequences obtained from lung cDNA library of normal person and cell line of AGZY83-a were similar in length but showed individual base difference. For OPB7-1, there is a low homogeneity to known gene by analysis in GenBank, but 3 contigs homologous to OPB7-1 were located at chromosome 1(1p31-1p34). Different degrees of expression were noted in tumor tissues from 24 cases of lung carcinoma, however no significant expression was found in their corresponding normal tissues. And high expression was found in the lung tissues of cases with lymph node metastasis.
Conclusion:
OPB7-1 may be a novel gene. It may be a tumor related gene in occurrence and metastasis of lung carcinoma.