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Updated: Aug 11, 2026
Chromosomics: Detection of Numerical and Structural Alterations in All 24 Human Chromosomes Simultaneously Using a Novel OctoChrome FISH Assay
Published on: February 6, 2012
[Detection of common chromosome abnormalities in myelodysplastic syndrome with a panel fluorescence in situ
Yongmei Shen1, Yongquan Xue, Jianyong Li
1First Affiliated Hospital of Soochow University, Jiangsu Institue of Hematology, Suzhou, Jiangsu, 215006 P. R. China. uujihsmc@public1.sz.js.cn
Objective:
To evaluate the value of a panel fluorescence in situ hybridization (FISH) in the detection of common chromosome abnormalities in myelodysplastic syndrome (MDS).
Methods:
Twenty cases of MDS patients, whose karyotypes were unknown by the FISH examiner beforehand, were analyzed with a panel FISH using YAC248F5 (5q31), YAC938G5 (7q32), CEP8 and YAC 912C3 (20q12) probes to detect the frequently occurring chromosome abnormalities (-5/5q, -/7q-, +8, 20q-) in MDS. Then the results were compared to those of conventional cytogenetics (CC).
Results:
Among 20 cases, 13 cases were found to carry common chromosome abnormalities by panel FISH (trisomy 8, five cases; -5/5q-, one case; 20q-, five cases; 5q- accompanying 20q-, one case; complex abnormalities, one case). However, on CC examination, only five cases were found to have common chromosomal abnormalities (20q-, four cases; 5q- accompanying 20q-, one case). In addition, trisomy 21, marker chromosome and complex abnormalities comprising -5, -7 and marker chromosomes were seen in one case each, the rest were normal.
Conclusion:
Panel FISH is a useful tool of molecular cytogenetics in the detection of common chromosome abnormalities in MDS.
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