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Related Experiment Videos

Experimental model to estimate intestinal viability using charge-coupled device microscopy.

M Yasumura1, Y Mori, H Takagi

  • 1First Department of Surgery, Gifu University School of Medicine, 40 Tsukasa, Japan. yasumi@cc.gifu-u.ac.jp

The British Journal of Surgery
|April 4, 2003
PubMed
Summary

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Charge-coupled device (CCD) microscopy offers new standards for assessing intestinal viability. Maximum velocity (Vmax) and S ratios effectively predict survival after strangulation release, aiding surgical decisions.

Area of Science:

  • Surgical assessment
  • Medical imaging technology

Background:

  • Intraoperative assessment of intestinal viability after strangulation release is challenging.
  • Charge-coupled device (CCD) microscopy offers a potential solution for evaluating intestinal viability.

Purpose of the Study:

  • To establish clinical standards for evaluating intestinal viability using CCD microscopy.
  • To determine the efficacy of Vmax ratio and S ratio as indicators of intestinal viability.

Main Methods:

  • A rat ileus model with induced strangulation (15-120 min) was utilized.
  • CCD microscopy captured images to calculate maximum velocity (Vmax) ratio and S ratio.
  • Rats were categorized into survival (group 1) and mortality (group 2) groups.

Main Results:

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  • Both Vmax ratio and S ratio were significantly higher in the survival group (P < 0.01).
  • Survival was observed with Vmax ratio ≥ 0.76 and S ratio ≥ 0.61.
  • Mortality occurred with Vmax ratio ≤ 0.54 and S ratio ≤ 0.51.

Conclusions:

  • Vmax ratio and S ratio serve as reliable indices for evaluating intestinal viability.
  • CCD microscopy provides objective metrics for intraoperative assessment of intestinal viability.