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Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages
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Visualization of Inflammatory Caspases Induced Proximity in Human Monocyte-Derived Macrophages

Published on: April 6, 2022

Dual effects of caspase-1, interleukin-1 beta, tumour necrosis factor-alpha and nerve growth factor receptor in

Z Mackiewicz1, M Hukkanen, D Povilenaite

  • 1Institute of Biomedicine/Anatomy, Biomedicum Helsinki, University of Helsinki, Finland.

Clinical and Experimental Rheumatology
|April 4, 2003
PubMed
Abstract

Insights

Pro-inflammatory factors like tumor necrosis factor-alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) are linked to muscle damage in myopathies. Low-level expression may aid skeletal muscle regeneration.

Area of Science:

  • Muscle biology
  • Immunology
  • Pathology

Background:

  • Myopathies, including inflammatory and non-inflammatory types, involve skeletal muscle degeneration.
  • Factors influencing muscle degeneration and regeneration are not fully understood across different myopathies.

Purpose of the Study:

  • To analyze the expression of key factors in skeletal muscle degeneration and regeneration.
  • To compare these factors across dermatomyositis (DM), systemic sclerosis (SSc), polymyositis (PM), systemic lupus erythematosus (SLE), and non-inflammatory myopathies.

Main Methods:

  • Immunohistochemical staining of skeletal muscle biopsies from 50 patients (10 per condition).
  • Analysis of tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), caspase-1, CD68, inducible nitric oxide synthase (NOS2), and nerve growth factor receptor (NGFR).
  • Utilized a TechMate staining robot and biotin-streptavidin protocol.

Main Results:

  • TNF-alpha, IL-1 beta, caspase-1, and NOS2 were expressed in dystrophic muscle fibers.
  • Low levels of TNF-alpha and IL-1 beta were observed near regenerating fibers in both inflammatory and non-inflammatory myopathies.
  • Caspase-1 expression was higher in non-inflammatory myopathies, suggesting a role in regeneration.

Conclusions:

  • Prominent expression of TNF-alpha, IL-1 beta, NOS2, and caspase-1 correlates with muscle fiber damage.
  • Low-level expression of these factors, along with NGFR, may contribute to muscle regeneration and healing.

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