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Related Experiment Videos

Retinal pigment epithelial function: a role for CFTR?

Sasha Blaug1, Richard Quinn, Judy Quong

  • 1School of Optometry, University of California, Berkeley, CA 94720-2020, USA.

Documenta Ophthalmologica. Advances in Ophthalmology
|April 5, 2003
PubMed
Summary

The cystic fibrosis transmembrane conductance regulator (CFTR) protein is present in human fetal retinal pigment epithelium (RPE). CFTR likely mediates the fast oscillation component of the electrooculogram (EOG) response.

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Area of Science:

  • Ophthalmology
  • Cell Biology
  • Physiology

Background:

  • The extracellular space between photoreceptors and retinal pigment epithelium (RPE) changes volume and composition with light.
  • The RPE exhibits slow voltage and resistance changes upon light onset.
  • The electrooculogram (EOG) measures two slow RPE responses: the fast oscillation (FO) and light peak.

Purpose of the Study:

  • To identify proteins involved in RPE transport pathways.
  • To investigate the role of these proteins in EOG responses.
  • To determine if CFTR is involved in the fast oscillation (FO) of the EOG.

Main Methods:

  • Reverse transcription-polymerase chain reaction (RT-PCR) on human fetal RPE.
  • Immunolocalization studies.

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  • Electrophysiological techniques.

    • Main Results:

    • The cystic fibrosis transmembrane conductance regulator (CFTR) was identified in human fetal RPE.
    • Evidence suggests CFTR is involved in mediating the fast oscillation (FO) component of the EOG.

    Conclusions:

    • CFTR is expressed in human fetal RPE.
    • CFTR plays a role, directly or indirectly, in the fast oscillation (FO) of the electrooculogram (EOG).