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Macroaffinity ligand-facilitated three-phase partitioning (MLFTPP) of alpha-amylases using a modified alginate
Kalyani Mondal1, Aparna Sharma, Lata Lata
1Chemistry Department, Indian Institute of Technology, Delhi Hauz Khas, New Delhi 110016, India.
Biotechnology Progress
|April 5, 2003
Abstract:
The crude extracts of alpha-amylases when mixed with alginate, tert-butyl alcohol, and ammonium sulfate resulted in an interfacial precipitate containing polymer-bound amylase. The precipitate was dissolved in 1 M maltose to recover alpha-amylase activity. The recovery of alpha-amylases were 74%, 77%, and 92% in the case of Bacillus amyloliquefaciens, wheat germ, and porcine pancreas, respectively. All purified preparations showed a single band on SDS-PAGE.