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Development and Functional Characterization of Murine Tolerogenic Dendritic Cells
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Bovine dendritic cells generated from monocytes and bone marrow progenitors regulate immunoglobulin production in

L M Pinchuk1, B L Boyd, E F Kruger

  • 1Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, P.O. Box 6100, 39762-6100, Mississippi State, MS, USA. pinchuk@cvm.msstate.edu

Comparative Immunology, Microbiology and Infectious Diseases
|April 5, 2003
PubMed

We examined whether bovine monocyte-derived and bone marrow (BM) dendritic cells (DCs) regulate antibody production in activated peripheral blood B cells. DCs were generated from monocytes and BM progenitors in the presence of bovine recombinant granulocyte/macrophage colony-stimulating factor (GM-CSF) and interleukin 4 (IL-4). Monocyte-derived DCs promoted B cells activated by the anti-CD3 triggered CD4(+) T cells or through immunoglobulin M (IgM) receptor to increase the level of IgG secretion. Furthermore, the addition of DCs triggered B cells activated through IgM receptors to produce IgG2 and IgA, thus inducing an isotype switch. BM-derived DCs increased the production of IgG in B cells activated by the anti-CD3 triggered CD4(+) T cells, but unlike monocyte-derived DCs did not have any effect on B cells activated through surface IgM. These data suggest that the regulation of humoral immune responses in cattle depends on the origin of DCs and the mode of B cell activation.

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