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Related Experiment Videos

Repeated fasting/refeeding elevates plasma leptin without increasing fat mass in rats.

Yong-Woon Kim1, Philip J Scarpace

  • 1Department of Veterans Affairs Medical Center, Gainesville, FL 32608-1197, USA. ywkim@med.yu.ac.kr

Physiology & Behavior
|April 5, 2003
PubMed
Summary

Repeated fasting and refeeding cycles in rats led to increased leptin levels and gene expression without significant weight gain. This suggests an adaptive hormonal response to altered feeding patterns, impacting energy homeostasis.

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Area of Science:

  • Metabolism and Endocrinology
  • Nutritional Science
  • Physiology

Background:

  • Repeated fasting and refeeding can enhance fat storage capacity in adipose tissue.
  • Rodent studies often show reduced weight gain in fasted/refed animals compared to controls.
  • Leptin, a hormone regulating energy balance, may be affected by repeated fasting and refeeding cycles.

Purpose of the Study:

  • To investigate the adaptive response of leptin to repeated cycles of 1-day fasting and 1-day refeeding over 42 days in rats.
  • To examine the impact of these cycles on body weight, food intake, leptin levels, and related gene expression.

Main Methods:

  • Rats underwent repeated fasting and refeeding (RFR) cycles for 42 days.
  • Body weight, daily food intake, circulating leptin levels, and leptin mRNA in retroperitoneal white adipose tissue (RT-WAT) were measured.

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  • Hypothalamic proopiomelanocortin (POMC) and RT-WAT lipoprotein lipase (LPL) mRNA levels were assessed.
  • Comparisons were made with control and pair-fed (PF) groups.

    • Main Results:

    • RFR rats exhibited reduced body weight gain and suppressed food intake after Day 16.
    • Circulating leptin levels and leptin mRNA in RT-WAT were significantly elevated in RFR rats.
    • Hypothalamic POMC gene expression was augmented in RFR rats.
    • No significant difference in visceral fat mass was observed; however, LPL mRNA levels increased in RT-WAT.

    Conclusions:

    • Chronic repeated fasting and refeeding up-regulates leptin without increasing adiposity.
    • Elevated leptin levels correlate with increased POMC gene expression, reduced food intake, and diminished body weight gain.
    • These findings highlight an adaptive metabolic and hormonal response to cyclical feeding patterns.