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Published on: October 23, 2012
Chitosan from Syncephalastrum racemosum used as a film support for lipase immobilization
R V S Amorim1, E S Melo, M G Carneiro-da-Cunha
1Laboratório de Imunopatologia Keizo Asami--LIKA and Departamento de Bioquímica, Universidade Federal de Pernambuco-UFPE, Av. Professor Moraes Rego s/n, Cidade Universitária, 50670-901 Recife, PE, Brazil. ramorim@dbm.ufpb.br
Abstract:
Chitosan from a native Mucoralean strain, Syncephalastrum racemosum, isolated from herbivorous dung (Northeast-Brazil), was used as a film support for lipase immobilization. S. racemosum showed highest chitosan yield (152 mg g dry mycelia weight(-1); 15.2% of dry mycelia weight) among the nine strains screened, which presented 89% D-glucosamine. A chitosan film was used for lipase (EC 3.1.1.3) immobilization using glutaraldehyde as a bifunctional agent. The immobilized lipase retained 47% (12.6 micromol s(-1) m(-2)) of its initial catalytic activity after four cycles of reaction. This result is comparable (same order of magnitude) to that of the enzyme immobilized on film made from commercially available crustacean chitosan.
