Search research articles

Search research articles

Related Experiment Videos

Stopped-flow fluorescence polarization immunoassay.

Agustina Gómez-Hens¹, M Paz Aguilar-Caballos

¹Department of Analytical Chemistry, Edificio C-3 (Anexo), Campus of Rabanales, University of Córdoba, E-14071 Córdoba, Spain. qa1gohea@uco.es

Combinatorial Chemistry & High Throughput Screening

|April 8, 2003

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Usefulness of magnetically-controlled MNPs-enzymes microreactors for the fluorimetric determination of total cholesterol in serum.

Talanta·2019

Same author

Automatic determination of coenzyme Q10 in food using cresyl violet encapsulated into magnetoliposomes.

Food chemistry·2016

Same author

Usefulness of palladium impregnated magnetite nanoparticles for polyphenol determination.

Talanta·2016

Same author

Automatic determination of polyphenols in wines using laccase and terbium oxide nanoparticles.

Food chemistry·2014

Same author

Fluorometric determination of alkaline phosphatase activity in food using magnetoliposomes as on-flow microcontainer devices.

Journal of agricultural and food chemistry·2014

Same author

Application of Tb(4)O(7) nanoparticles for lasalocid and salicylate determination in food analysis.

Journal of agricultural and food chemistry·2012

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

This study introduces a kinetic approach to fluorescence polarization immunoassay (FPIA) using stopped-flow mixing. This method measures reaction rates, offering a novel analytical parameter for enhanced screening applications.

Area of Science:

Analytical Chemistry
Biochemistry

Background:

Conventional fluorescence polarization immunoassay (FPIA) relies on equilibrium signals.
Kinetic methods offer alternative analytical parameters for immunoassays.

Purpose of the Study:

To present a general survey of the analytical applications of kinetic methodology in FPIA.
To introduce and evaluate the stopped-flow mixing technique (SF) coupled with FPIA (SF-FPIA).

Main Methods:

Utilizing stopped-flow mixing (SF) to capture the initial reaction rate between antibody and tracer.
Employing the initial reaction rate as the analytical parameter, replacing the equilibrium signal.
Describing the necessary instrumentation for SF-FPIA.

Main Results:

Related Experiment Videos

SF-FPIA provides an alternative analytical parameter based on reaction kinetics.
Comparison of SF-FPIA features with conventional FPIA is presented.
Demonstration of the potential for routine screening in various analytical fields.

Conclusions:

SF-FPIA offers a viable kinetic approach to fluorescence polarization immunoassay.
The method shows promise for routine screening in clinical, environmental, and food analysis.
Kinetic analysis provides a valuable alternative to equilibrium measurements in FPIA.