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Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

The use of fluorescence polarization assays for the detection of infectious diseases

Michael E Jolley1, Mohammad S Nasir

  • 1Diachemix LLC, 683 E. Center Street, Unit H, Grayslake, IL 60030, USA. mickj@diachemix.com

Combinatorial Chemistry & High Throughput Screening
|April 8, 2003
PubMed

Insights

Fluorescence Polarization Assays (FPAs) offer a versatile method for detecting infectious diseases. This technique utilizes O-polysaccharides and other biomolecules for sensitive antibody and bacterial cell detection.

Area of Science:

  • Biochemistry
  • Immunology
  • Microbiology

Background:

  • Fluorescence Polarization Assays (FPAs) are valuable tools for infectious disease diagnostics.
  • Various biomolecules, including O-polysaccharides (OPSs), proteins, and peptides, can be employed in FPAs.

Purpose of the Study:

  • To demonstrate the broad applicability of FPAs in detecting infectious agents and related antibodies.
  • To highlight the sensitivity and speed of FPAs and related techniques.

Main Methods:

  • Utilizing O-polysaccharides (OPSs) for antibody detection against Gram-negative bacteria (e.g., Brucella spp., Salmonella spp.) in various sample types.
  • Employing proteins and peptides for detecting Mycobacterium bovis and Equine Infectious Anemia Virus.
  • Applying Fluorescence Polarization Inhibition Assays (FPIAs) for Salmonella spp. cell detection and quantitation.
  • Integrating Strand Displacement Amplification (SDA) with FP for rapid, single-cell detection of enterohemorrhagic E. coli (EHECS).

Main Results:

  • Successful detection of antibodies in serum, milk, and blood using OPSs.
  • Demonstrated detection of Mycobacterium bovis and Equine Infectious Anemia Virus.
  • Achieved specific and sensitive detection and quantitation of Salmonella spp. using FPIAs.
  • Reported single-cell level detection of EHECS within 30 minutes using SDA-FP.

Conclusions:

  • FPAs are highly adaptable for detecting a range of infectious diseases and pathogens.
  • The integration of amplification techniques like SDA enhances the sensitivity and speed of FP-based diagnostics.
  • FPAs offer a promising platform for rapid and sensitive infectious disease screening.

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