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[Identification of multidrug resistance related genes in leukemia by suppression subtractive hybridization]
Ning-xi Zhu1, Shu Zheng, Rong-zhen Xu
1Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou 310006, China.
Objective:
To clone and screen genes related to multidrug resistance (MDR) in leukemia.
Methods:
Suppression subtractive hybridization (SSH) was performed to profile differentially expressed genes between a MDR leukemia cell line (K562/DOX, as tester) and its parent cell line (K562, as driver). Reverse Northern dot blot was carried out to further screen the subtracted cDNA library. The overexpressed cDNA fragments in K562/DOX cells were sequenced and compared with known genes in Genbank. RT-PCR and Northern blot were employed to confirm the differential expression of some identified genes.
Results:
Eleven genes were identified being overexpressed in K562/DOX, including S3 ribosomal protein (S3rp) gene, NADH dehydrogenase subunit 2 (ND2) gene and My023 gene, which have not been reported to be related to MDR in cancer.
Conclusion:
Several genes, which might be involved in MDR were identified, indicating novel mechanisms of MDR in leukemia.