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Updated: Sep 26, 2026
Analysis of Epididymal Protein Synthesis and Secretion
Published on: August 25, 2018
[Molecular cloning of human sperm surface protein P34H gene and semi-quantitative analysis of its expression in
Xin-Yi Xia1, Xiao-Feng Xu, Yun Gao
1College of Life Science, Nanjing Normal University, Nanjing, Jiangsu 210097, China.
Objectives:
To identify, clone and sequence human sperm protein P34H gene and perform semi-quantitative analysis of its expression in testis and epididymidis.
Methods:
Total RNA was prepared from human epididymidis tissue. cDNA fragment encoding human P34H was amplified by RT-PCR using specific primers, and then was cloned into pGEM-T vector. Inserted P34H gene was sequenced by ABI 377 DNA Sequencer. Meanwhile, semi-quantitative analysis of P34H expression in testis and caput, corpus, cauda epididymidis was done by RT-PCR based on beta-actin as an inner control.
Results:
Human sperm protein P34H gene was successfully cloned and its cDNA sequence was submitted to GenBank(Accession No. AF515625).
Conclusions:
The cloning of P34H gene may be useful for the further basic and clinical studies on P34H.
