Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Application of a microchamber array for DNA amplification using a novel dispensing method.

Yasutaka Matsubara1, Masaaki Kobayashi, Yasutaka Morita

  • 1The School of Materials Science, Japan Advanced Institute of Science and Technology, Tatsunokuchi, Ishikawa, Japan. yasutaka@jaist.ac.jp

Archives of Histology and Cytology
|April 12, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Urgent Resection of Hepatocellular Carcinoma with Intracardiac Extension under Moderate Hypothermic Circulatory Arrest.

Interdisciplinary cardiovascular and thoracic surgery·2026
Same author

Report From the 32nd Annual Congress of the Japanese Society for Aeromedical Services.

Air medical journal·2026
Same author

Emergence of norovirus GII.7 and predominance of recombinant strains in pediatric acute gastroenteritis in Japan, 2021-2024.

Archives of virology·2026
Same author

Co-Circulation and Mixed Infections of Classic and Melbourne Human Astroviruses Among Pediatric Acute Gastroenteritis Patients in Japan, 2018-2024.

Journal of medical virology·2026
Same author

A novel prohibitin inhibitor acts as a dual inhibitor of angiogenesis and fibrosis.

Biochemical and biophysical research communications·2026
Same author

Tiled-Amplicon Whole-Genome Sequencing Method Reveals Endemic Circulation of Human Adenovirus Type 3 in Japan.

Viruses·2026

This study improved a microchamber array chip for DNA amplification. The new design uses an oil layer for easier sample introduction and product retrieval, enhancing DNA analysis capabilities.

Area of Science:

  • Biotechnology
  • Microfluidics
  • Molecular Biology

Background:

  • Microchamber array chips enable DNA amplification using semiconductor microfabrication.
  • Previous designs sealed chips, hindering product retrieval for further analysis.

Purpose of the Study:

  • To develop improved methods for covering microchambers and introducing reaction solutions in a microchamber array chip.
  • To overcome limitations in product retrieval for enhanced DNA analysis.

Main Methods:

  • Fabricated a microchamber array chip with an oil layer instead of a cover glass slip.
  • Introduced DNA amplification solutions into chambers via the oil layer using a nano-liter dispenser.
  • Utilized thermal cycling for DNA amplification and fluorescence microscopy for detection.

Related Experiment Videos

Main Results:

  • Successfully coated the chip with an oil layer, facilitating sample introduction.
  • Enabled easy retrieval of amplified DNA products using a micromanipulator.
  • Demonstrated a functional system for DNA amplification and subsequent analysis.

Conclusions:

  • The improved microchamber array chip design enhances sample manipulation and product retrieval.
  • This system offers a more accessible platform for DNA amplification and downstream applications.
  • The oil layer method provides a versatile solution for microfluidic device design.