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Updated: Sep 26, 2026

Large-scale Production of Recombinant RNAs on a Circular Scaffold Using a Viroid-derived System in Escherichia coli
Published on: November 30, 2018
A new structural motif in the left terminal domain of large viroids identified by covariation analysis
1Institut für Anorganische und Analytische Chemie, Justus-Liebig-Universität Giessen, Schuberstrasse 60, D-35392 Giessen, Germany. gast@gesim.de
Abstract:
Sequence alignment of non-hammerhead viroids (Pospiviroidae) reveals that they can be divided into large and small viroids. Covariation analysis of these groups provides evidence for a rod-like secondary structure, but also for two proposed suboptimal structures, namely hairpin I and a bifurcation in the left terminus of large viroids. The strongest covariation within the branched T1 domain of all large Pospiviroidae indicates a new tertiary interaction, resulting in a double-pseudoknot within the (+) strand or an additional hairpin in the (-) strand. The structural element is expected to play a role in the viroid's life cycle.
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