Related Experiment Videos
NMR and modelling studies of disaccharide conformation
Norman W H Cheetham1, Paramita Dasgupta, Graham E Ball
1School of Chemical Sciences, The University of New South Wales, Sydney 2052, Australia. n.cheetham@unsw.edu.au
Carbohydrate Research
|April 12, 2003
Abstract:
Long-range heteronuclear coupling constants were measured across the glycosidic linkages for a series of eight alpha- or beta-linked disaccharides in aqueous solution. Multiple 13C site-selective excitation experiments using 1H decoupling in conjunction with pulsed field gradient-enhanced spectroscopy were used to determine 3J(C,H) values. These were subsequently compared with the respective couplings calculated, using a Karplus relationship, from molecular dynamics simulations with the explicit inclusion of water.