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Detection of single cells of Cryptococcus neoformans in clinical samples by solid-phase cytometry
T G M Bauters1, D Swinne, V Stove
1Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium.
Journal of Clinical Microbiology
|April 19, 2003
Abstract:
A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.