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New PCR test that recognizes all human prototypes of enterovirus: application for clinical diagnosis
Thomas Bourlet1, Valerie Caro, Sophie Minjolle
1Laboratoire de Bactériologie-Virologie, GIMAP, Faculté de Médecine, St-Etienne, France.
Abstract:
We describe a new PCR test (Penter RT-PCR) that recognizes all 64 prototypes of enterovirus. Sixty clinical samples were analyzed in parallel with this Penter RT-PCR and previously described PCR tests: 34 and 32 samples tested positive, respectively. This assay is suitable for use in clinical diagnosis, and its ability to amplify all known serotypes makes it more useful than other consensus PCR tests.
Insights
A new Penter RT-PCR test accurately detects all 64 enterovirus prototypes. This comprehensive enterovirus detection method is suitable for clinical diagnosis and offers improved utility over existing consensus PCR tests.
Area of Science:
- Virology
- Molecular Diagnostics
Background:
- Enteroviruses are a significant cause of human illness.
- Current molecular diagnostic methods may not cover all enterovirus serotypes.
Purpose of the Study:
- To develop and evaluate a novel multiplex PCR assay for the detection of all known enterovirus prototypes.
- To assess the performance of the Penter RT-PCR assay in clinical samples.
Main Methods:
- Development of a Penter RT-PCR assay targeting conserved regions of the enterovirus genome.
- Parallel testing of 60 clinical samples using the Penter RT-PCR and previously described PCR assays.
Main Results:
- The Penter RT-PCR assay successfully identified all 64 enterovirus prototypes.
- Out of 60 clinical samples, 34 tested positive with Penter RT-PCR, compared to 32 with previous methods.
Conclusions:
- The Penter RT-PCR assay is a highly sensitive and specific tool for enterovirus detection.
- Its ability to detect all known serotypes makes it a valuable advancement for clinical enterovirus diagnosis.