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drs (Distantly related sic) gene polymorphisms among emm12-type Streptococcus pyogenes isolates
Claudia M Brandt1, Gerhard Haase, Barbara Spellerberg
1Institute of Medical Microbiology and National Reference Center for Streptococci, University Hospital Aachen, 52057 Aachen, Germany. claudia.brandt@em.uni-frankfurt.de
Journal of Clinical Microbiology
|April 12, 2003
Summary
Genetic typing of Streptococcus pyogenes emm12 isolates revealed novel drs alleles. Sequencing drs genes may help differentiate strains with similar vir regulon patterns, aiding in understanding invasive bacterial infections.
Area of Science:
- Microbiology
- Genetics
- Infectious Diseases
Background:
- Streptococcus pyogenes is a significant human pathogen causing various infections.
- The emm12 type is associated with specific clinical presentations.
- Genetic variability within S. pyogenes strains influences disease epidemiology and treatment.
Purpose of the Study:
- To genetically characterize emm12-type Streptococcus pyogenes isolates.
- To investigate the utility of sequencing drs genes for strain differentiation.
- To explore potential associations between drs alleles and vir regulon patterns.
Main Methods:
- Genetic typing of 28 emm12-type Streptococcus pyogenes isolates.
- Sequencing of the drs (distantly related sic) genes.
- Analysis of vir regulon restriction patterns.
Main Results:
- Identification of two novel drs alleles and polymorphism similar to sic.
- No significant association found between the five drs alleles and five vir regulon patterns.
- Drs gene sequencing showed potential for differentiating S. pyogenes isolates.
Conclusions:
- Drs gene sequencing offers a valuable tool for high-resolution typing of emm12 S. pyogenes.
- This method can further differentiate strains that are indistinguishable by vir regulon analysis.
- Enhanced strain differentiation aids in epidemiological studies of Streptococcus pyogenes infections.