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Updated: Sep 26, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
A new minor groove binding asymmetric cyanine reporter dye for real-time PCR
Martin Bengtsson1, H Jonas Karlsson, Gunnar Westman
1Department of Chemistry and Bioscience, Chalmers University of Technology 41296 Göteborg and TATAA Biocenter, Medicinaregatan 9E, 413 90 Göteborg, Sweden.
Abstract:
The minor groove binding asymmetric cyanine dye 4-[(3-methyl-6-(benzothiazol-2-yl)-2,3-dihydro- (benzo-1,3-thiazole)-2-methylidene)]-1-methyl-pyridin ium iodide (BEBO) is tested as sequence non- specific label in real-time PCR. The fluorescence intensity of BEBO increases upon binding to double-stranded DNA allowing emission to be measured at the end of the elongation phase in the PCR cycle. BEBO concentrations between 0.1 and 0.4 micro M generated sufficient fluorescence signal without inhibiting the PCR. A comparison with the commonly used reporter dye SYBR Green I shows that the two dyes behave similarly in all important aspects.
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