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Updated: Aug 19, 2026

Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
Published on: October 29, 2009
The electrophoretic mobility of double-stranded RNA in polyacrylamide gels as a function of molecular weight
Abstract:
A re-evaluation of the mobility of double-stranded RNA on polyacrylamide gels over a molecular weight range of 0.46-6.3 . 10(6) was carried out using double-stranded RNAs of: bacteriophage ø6; virus like particles or mycoviruses of Penicillium chyrsogenum, Penicillium stoloniferum and Helminthosporium maydis, and reovirus type III. When the relative mobility on polyacrylamide gels was plotted as a function of log molecular weight, a smooth curve could be drawn which passed through all points. The implications of these findings to the determination of molecular weight of double-stranded RNA by polyacrylamide gel electrophoresis are discussed.
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