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Updated: Sep 21, 2026

Isolation and Culture of Primary Mouse Keratinocytes from Neonatal and Adult Mouse Skin
Published on: July 14, 2017
Determination of leptin signaling pathways in human and murine keratinocytes
Itamar Goren1, Josef Pfeilschifter, Stefan Frank
1Pharmazentrum Frankfurt, Institut für Allgemeine Pharmakologie und Toxikologie, Klinikum der Johann Wolfgang Goethe-Universität Frankfurt am Main, Theodor-Stern-Kai 7, Germany.
Abstract:
Recently, we have determined the role of leptin as a keratinocyte mitogen in vitro and during skin repair in vivo. In this study, we assessed leptin-stimulated signal transduction in the human keratinocyte cell line HaCaT and the murine keratinocyte cell line PAM 212. HaCaT keratinocytes were characterized by a constitutive phosphorylation of janus kinase (JAK)-2. By contrast, PAM 212 keratinocytes responded to leptin with a rapid phosphorylation of JAK-2. However, we could determine a cytoplasmic activation of signal transducer and activator of transcription (STAT)-3 by phosphorylation of tyrosine 705 (Y705) within minutes only upon leptin stimulation in both keratinocyte cell lines. Subsequently, STAT-3 translocated to the nucleus where serine 727 (S727) was phosphorylated, establishing a transcriptionally active STAT-3 transcription factor. In a model of cutaneous wound healing, treatment of leptin-deficient obese/obese (ob/ob) mice strongly augmented phosphorylation of STAT-3 (Y705) in wound keratinocytes also in vivo.
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