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Updated: Sep 26, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Quantitative bioanalysis of enantiomeric drugs using capillary electrophoresis and electrospray mass spectrometry
Erick K Kindt1, Stephen Kurzyniec, Shau-Chun Wang
1Bioanalytical Core Group, Pfizer Global R&D, 2800 Plymouth Road, Ann Arbor, MI 48105, USA. erick.kindt@pfizer.com
Abstract:
A novel assay method for an enantiomeric pair of drugs has been developed using a combination of capillary electrophoresis and electrospray tandem mass spectrometry connected with a homemade interface. Accurate quantification was demonstrated in plasma from 0.25 to 50 microg/ml. A liquid-liquid sample preparation technique allowed improvement in the quantitation limit to 10 ng/ml. Variables for the enantiomeric separation, including chiral selective reagent, organic solvents, buffer and acid concentration as well as injection technique, were optimized. This assay proved adequate for analysis of neat, spiked plasma, and plasma from a pharmacological study of the drug enantiomers.
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