Related Experiment Video
Updated: May 6, 2026

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
Published on: January 5, 2015
UV-visible and fluorescence spectral study on a pH controlled transfer process of an amphiphilic porphyrin in
1Institute of Mesocopic Solid State Chemistry and National Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, People's Republic of China. linguo@jlonline.com
Abstract:
5,15-di(4-hydroxyphenyl)-10,20-di(hexadecyloxyphenyl) porphyrin P was solubilized in nonionic polyoxyethylene(9.5) octylphenol (Triton X-100 or TX-100) micelle solutions. By means of analyzing the UV-visible and fluorescence spectra of the synthesized amphiphilic porphyrin P in different solvent environments, and the relationship between the solubilizing location of the porphyrins in TX-100 micelle and the microenvironment polarity, P is shown to involve in a transfer process for the porphyrin moiety from inner to the outer surface of TX-100 micelle as the pH is increased. The kinetic study of porphyrin incorporate with Cu(II) shows that metalation rate of porphyrin increases with the pH increasing, indicating that metalation rate could be controlled by changing pH.
More Related Videos
10:08Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
Published on: October 24, 2017
12:15Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Published on: February 21, 2019
Related Concept Videos
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is...
UV–Vis Spectrometers
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Variables Affecting Phosphorescence and Fluorescence
Photoluminescence: Applications