Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Noise in eukaryotic gene expression.

William J Blake1, Mads KAErn, Charles R Cantor

  • 1Center for BioDynamics, Center for Advanced Biotechnology, Bioinformatics Program, and Department of Biomedical Engineering, Boston University, 44 Cummington Street, Boston, Massachusetts 02215, USA.

Nature
|April 11, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

KatG catalase deficiency confers bedaquiline hyper-susceptibility to isoniazid resistant <i>Mycobacterium tuberculosis</i>.

bioRxiv : the preprint server for biology·2023
Same author

Controlled Continuous Evolution of Enzymatic Activity Screened at Ultrahigh Throughput Using Drop-Based Microfluidics.

Angewandte Chemie (International ed. in English)·2023
Same author

Correction: High-resolution DNA size enrichment using a magnetic nano-platform and application in non-invasive prenatal testing.

The Analyst·2022
Same author

Efficient detection and post-surgical monitoring of colon cancer with a multi-marker DNA methylation liquid biopsy.

Proceedings of the National Academy of Sciences of the United States of America·2021
Same author

Artificial intelligence, drug repurposing and peer review.

Nature biotechnology·2020
Same author

ANERGY TO SYNERGY-THE ENERGY FUELING THE RXCOVEA FRAMEWORK.

International journal for multiscale computational engineering·2020
Same journal

Incoming US science academy chief vows to 'double down' on research.

Nature·2026
Same journal

Author Correction: Synthesis of enantioenriched atropisomers by biocatalytic deracemization.

Nature·2026
Same journal

Electrodeposited self-assembled molecules for perovskite photovoltaics.

Nature·2026
Same journal

Neutrino's nursery found: the 'Shadow Blaster'.

Nature·2026
Same journal

Dementia risk in middle-aged people linked to a blood protein.

Nature·2026
Same journal

Daily briefing: What's really happening with trust in science.

Nature·2026
See all related articles

Eukaryotic gene expression is noisy, with transcription driving cell-to-cell variability. This transcriptional noise, unlike in prokaryotes, can be controlled at the translational level and impacts cellular differentiation.

Area of Science:

  • Molecular Biology
  • Systems Biology
  • Genetics

Background:

  • Gene expression in eukaryotes is quantal and probabilistic, leading to cell heterogeneity.
  • Stochasticity in gene expression is a key factor in individual cell responses to stimuli.
  • Understanding noise sources is crucial for explaining cellular variability.

Purpose of the Study:

  • To investigate the contribution of transcriptional stochasticity to cell-cell heterogeneity in eukaryotes.
  • To determine if transcriptional noise can be modulated at the translational level.
  • To explore the role of noise in gene regulatory networks and cellular differentiation.

Main Methods:

  • Utilized a stochastic model of eukaryotic transcription initiation.
  • Analyzed pulsatile messenger RNA (mRNA) production and reinitiation.

Related Experiment Videos

  • Studied noise propagation in a gene cascade network.
  • Conducted experimental validation in Saccharomyces cerevisiae.

    • Main Results:

    • Transcriptional stochasticity significantly contributes to eukaryotic cell-cell heterogeneity, differing from prokaryotes.
    • Pulsatile mRNA production via reinitiation is critical for noise dependence on transcriptional efficiency.
    • Noise can be modulated at the translational level.
    • Increased transcriptional noise in regulatory proteins amplifies cell-cell variability and prolongs bistable expression states.

    Conclusions:

    • Transcriptional noise is a major driver of heterogeneity in eukaryotic clonal populations.
    • Eukaryotic noise mechanisms, particularly pulsatile transcription, differ fundamentally from prokaryotes.
    • Modulating noise at the translational level offers a control mechanism.
    • Transcriptional noise plays a significant role in phenotypic variation and cellular differentiation processes.