Kinetics of Apoptosis of Peripheral Blood Leukocytes in Normal State and in Children Recovered from Acute...

The original method is proposed for the study of apoptosis kinetics in cell populations by determination of apoptotic cell accumulation during the day's incubation in serum free medium. We have studied the features of this process for peripheral blood granulocytes and lymphocytes from healthy children. High sensitivity of the proposed method has been shown for the estimation of apoptosis, taking for illustration the analysis of delayed chemotherapy influence on blood cells in children, recovered from acute limphoblastic leukemia (ALL). In children with ALL, the delayed myelosuppressive effect of antileukemic therapy has been proved to be not only in relation to the increase of blood cell ability to spontaneous apoptosis, but also to induce the certain impairments in the kinetics of this process.