Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Vasoformative sarcomas arising from BALB/3T3 cells attached to solid substrates.

C W Boone, N Takeichi, M Paranjpe

    Cancer Research
    |May 1, 1976
    PubMed
    Summary

    BALB/3T3 cells, typically non-tumorigenic, form vasoformative sarcomas when implanted with a substrate. These tumors exhibit altered growth properties and unique rejection antigens, indicating preneoplastic potential.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Physicochemical characterization of sildenafil-loaded solid lipid nanoparticle dispersions (SLN) for pulmonary application.

    International journal of pharmaceutics·2014
    Same author

    In vitro and ex vivo toxicological testing of sildenafil-loaded solid lipid nanoparticles.

    Inhalation toxicology·2013
    Same author

    Successful cochlear implantation in a patient with mitochondrial hearing loss and m.625G>A transition.

    The Journal of laryngology and otology·2011
    Same author

    Diagnostic-significance of liver-failure after newly-appeared serum fractions following liver surgery.

    Oncology reports·2011
    Same author

    Can breast cancer be prevented?

    Annals of Saudi medicine·2007
    Same author

    Discharge of monkey nucleus reticularis tegmenti pontis neurons changes during saccade adaptation.

    Journal of neurophysiology·2005

    Area of Science:

    • Cell Biology
    • Oncology
    • Cancer Research

    Background:

    • BALB/3T3 cells are a standard non-tumorigenic mouse embryo fibroblast line.
    • These cells exhibit contact inhibition, anchorage dependence, and high serum requirements.
    • They are often used as a control for studying tumorigenic cell lines.

    Purpose of the Study:

    • To investigate the in vivo tumorigenic potential of BALB/3T3 cells.
    • To characterize the histological and biological properties of tumors derived from BALB/3T3 cells.
    • To determine if BALB/3T3 cells possess preneoplastic characteristics.

    Main Methods:

    • Subcutaneous implantation of BALB/3T3 cells attached to polycarbonate platelets or glass beads in mice.
    • Histological and immunohistochemical analysis of tumor tissues.

    Related Experiment Videos

  • Assessment of tumor cell growth properties (proliferation, anchorage dependence) in vitro.
  • Transplantation studies to confirm tumor cell origin and investigate rejection antigens.
  • Analysis for endogenous oncornavirus induction.

    • Main Results:

    • BALB/3T3 cells formed vasoformative sarcomas within 2-3 months when implanted with a substrate.
    • Tumors showed vascular channel formation, suggesting endothelial cell origin.
    • Tumor cells lost postconfluence inhibition of proliferation and anchorage dependence.
    • Tumors exhibited unique transplantation rejection antigens.
    • Endogenous oncornavirus induction was observed in one of four tumors.

    Conclusions:

    • BALB/3T3 cells are preneoplastic and can spontaneously transform in vivo.
    • Implantation on a solid substrate is crucial for tumor formation.
    • The resulting tumors are derived from BALB/3T3 cells and display altered neoplastic properties.
    • Spontaneously transformed clones bear unique tumor rejection antigens.