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Recording of Inward Rectifying K+ Currents in Freshly Isolated Basilar Artery Smooth Muscle Cells by Patch Clamp Technique07:19

Recording of Inward Rectifying K+ Currents in Freshly Isolated Basilar Artery Smooth Muscle Cells by Patch Clamp Technique

This protocol describes a rapid and efficient method for isolating smooth muscle cells from the rat basilar artery and recording inward rectifying potassium channel currents in these cells using the whole-cell patch clamp technique. It offers a novel approach for researchers studying the basilar artery and ion...
715
Traction Cytometry Assay: A Method to Quantify Traction Force Exerted by Cancer Cells on ECM02:44

Traction Cytometry Assay: A Method to Quantify Traction Force Exerted by Cancer Cells on ECM

This video describes the traction force microscopy assay. The assay determines the cellular traction of human colon cancer cells on the extracellular matrix present on soft elastic hydrogel having embedded fluorescent beads. The resultant displacement of fluorescent beads is measured using an appropriate...
1.6K
Pattern Generation for Micropattern Traction Microscopy09:26

Pattern Generation for Micropattern Traction Microscopy

We describe improvements to a standard method for measuring cellular traction forces, based on microcontact printing with a single subtractive patterning step of dot arrays of extracellular matrix proteins on soft hydrogels. This method allows for simpler and more consistent fabrication of island patterns, essential for controlling cell cluster...
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Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy12:26

Control of Cell Adhesion using Hydrogel Patterning Techniques for Applications in Traction Force Microscopy

Near-UV lithography and traction force microscopy are combined to measure cellular forces on micropatterned hydrogels. The targeted light-induced release of single cells enables a high number of measurements on the same...
6.3K
Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration10:53

Traction Microscopy Integrated with Microfluidics for Chemotactic Collective Migration

Collective cell migration in development, wound healing, and cancer metastasis is often guided by the gradients of growth factors or signaling molecules. Described here is an experimental system combining traction microscopy with a microfluidic system and a demonstration of how to quantify the mechanics of collective migration under biochemical...
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Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform08:10

Fabrication and Implementation of a Reference-Free Traction Force Microscopy Platform

This protocol provides instructions for implementing multiphoton lithography to fabricate three-dimensional arrays of fluorescent fiducial markers embedded in poly(ethylene glycol)-based hydrogels for use as reference-free, traction force microscopy platforms. Using these instructions, measurement of 3D material strain and calculation of cellular tractions is simplified to promote high-throughput traction force...
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