Related Experiment Videos
Phytases: microbial sources, production, purification, and potential biotechnological applications
Ashima Vohra1, T Satyanarayana
1Department of Microbiology, University of Delhi, South Campus, Delhi 110 021, India.
Critical Reviews in Biotechnology
|April 16, 2003
Summary
This review covers phytase-producing microbes and optimal production conditions. It explores phytase purification, characteristics, and biotechnological uses in animal feed and food industries.
Area of Science:
- Biotechnology
- Enzymology
- Microbiology
Background:
- Phytases are crucial enzymes for breaking down phytate, an anti-nutritional factor in plant-based feeds.
- Understanding phytase production and application is vital for improving nutrient bioavailability in animal nutrition and food processing.
Purpose of the Study:
- To review microorganisms that produce phytase.
- To discuss optimal conditions for phytase production and purification methods.
- To explore biotechnological applications of phytases.
Main Methods:
- Literature review of scientific publications on phytase-producing microorganisms.
- Analysis of methods for phytase purification and characterization.
- Examination of heterologous gene expression and large-scale production strategies.
Main Results:
- Identification of various microorganisms capable of phytase production.
- Discussion of optimal parameters influencing phytase yield and activity.
- Overview of diverse purification techniques and enzyme properties.
- Exploration of advanced methods like heterologous gene expression for enhanced production.
Conclusions:
- Phytase production can be optimized through careful selection of microorganisms and controlled fermentation conditions.
- Effective purification strategies are essential for obtaining high-activity phytases.
- Biotechnological applications of phytases offer significant potential for the animal feed and food industries, promoting sustainability and efficiency.