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Related Experiment Videos

Analysis of plasmid samples on a microchip.

Li Ding1, Kathi Williams, Walter Ausserer

  • 1Caliper Technologies Corp., 605 Fairchild Drive, Mountain View, CA 94043-2234, USA. lding@arctur.com

Analytical Biochemistry
|April 16, 2003
PubMed
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A new LabChip assay on the Agilent 2100 Bioanalyzer rapidly analyzes plasmid DNA. This method accurately determines plasmid size, concentration, and isoforms, offering a valuable tool for molecular biology research.

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Plasmid DNA analysis is crucial for molecular biology.
  • Accurate determination of plasmid size, concentration, and isoforms is essential for various applications.
  • Existing methods can be time-consuming or lack resolution.

Purpose of the Study:

  • To develop and validate a novel LabChip-based assay for comprehensive plasmid DNA analysis.
  • To assess the assay's performance in terms of speed, accuracy, and resolution.
  • To demonstrate the utility of the assay for characterizing plasmid samples.

Main Methods:

  • Utilized the Agilent 2100 Bioanalyzer with a LabChip platform.
  • Developed a plasmid assay capable of analyzing multiple samples simultaneously.

Related Experiment Videos

  • Employed a supercoiled DNA sizing standard (2-16kb) for accurate size determination.
  • Optimized automated runs for a 30-minute analysis time.

    • Main Results:

    • The assay accurately determines plasmid sizes (2-8kb) with resolution better than 1kb.
    • Achieved high precision for molecular weight (MW) determination (CV <5%, error <5%).
    • Demonstrated separation of topological isoforms and semi-quantitative measurement of supercoiled plasmid concentration (1-100ng/µL, CV <20%).

    Conclusions:

    • The developed LabChip assay provides a rapid, accurate, and high-resolution method for plasmid DNA analysis.
    • The assay is suitable for determining multiple forms of plasmid DNA, including size, concentration, and isoforms.
    • This automated assay offers significant advantages for molecular biology workflows requiring efficient plasmid characterization.