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Related Experiment Videos

Optimization of a formulation containing viable lactic acid bacteria.

M Stadler1, H Viernstein

  • 1Institute of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090, Vienna, Austria.

International Journal of Pharmaceutics
|April 16, 2003
PubMed
Summary

Developing gastric juice resistant tablets for lactic acid bacteria (LAB) is crucial for probiotic efficacy. Optimal formulations using hydroxypropylmethylcellulose acetate succinate (HPMCAS) enhance LAB survival in acidic conditions.

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Area of Science:

  • Pharmaceutical Technology
  • Microbiology

Background:

  • Probiotics, including lactic acid bacteria (LAB), require protection from gastric acidity to ensure viable delivery to the intestines.
  • Developing effective delivery systems is essential for maximizing the therapeutic benefits of LAB probiotics.

Purpose of the Study:

  • To develop gastric juice resistant tablet formulations for lactic acid bacteria (LAB).
  • To optimize these formulations for enhanced LAB survival in acidic environments and controlled disintegration in intestinal fluid.

Main Methods:

  • Tablets were formulated using hydroxypropylmethylcellulose acetate succinate (HPMCAS), alginates, apple pectin, and Metolose as matrix components.
  • Formulations were optimized by varying LAB content, excipient amounts, and compaction forces, with survival rate in acid and disintegration time as key parameters.

Related Experiment Videos

  • Resistance to artificial gastric juice and disintegration in phosphate buffer (pH 6.8) were evaluated.

    • Main Results:

    • Hydroxypropylmethylcellulose acetate succinate (HPMCAS) content was found to correlate positively with gastric juice resistance.
    • Optimal formulations achieved a survival rate of not more than one log unit decrease after 2 hours in acid medium.
    • Slight amounts of HPMCAS were preferred to balance gastric resistance and reduce disintegration time in intestinal fluid.

    Conclusions:

    • Tablets formulated with a combination of LAB, HPMCAS, and sodium alginate demonstrated the best protective qualities against artificial gastric juice.
    • The study successfully developed gastric juice resistant LAB formulations, enhancing probiotic viability and efficacy.