Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Spatial and temporal pattern of Wnt-6 expression during chick development.

Marc Rodríguez-Niedenführ1, Verena Dathe, Heinz Jürgen Jacob

  • 1Institute of Anatomy II, University of Freiburg, Albertstrasse 17, 79104 Freiburg, Germany.

Anatomy and Embryology
|April 16, 2003
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of the abdominal musculature in the chicken embryo.

Journal of anatomy·2026
Same author

Development of ribs and intercostal muscles in the chicken embryo.

Journal of anatomy·2022
Same author

Regulation of Translation, Translocation, and Degradation of Proteins at the Membrane of the Endoplasmic Reticulum.

International journal of molecular sciences·2022
Same author

Regulation of calcium homeostasis and flux between the endoplasmic reticulum and the cytosol.

The Journal of biological chemistry·2022
Same author

The function of the co-chaperone ERdj4 in diverse (patho-)physiological conditions.

Cellular and molecular life sciences : CMLS·2021
Same author

Dual topology of co-chaperones at the membrane of the endoplasmic reticulum.

Cell death discovery·2021
Same journal

Online First publication.

Anatomy and embryology·2014
Same journal

The postnatal development of the optic nerve of a reptile (Vipera aspis): A quantitative ultrastructural study.

Anatomy and embryology·2006
Same journal

Immunophenotyping and spatio-temporal distribution of aortic cell clusters in the bovine embryo.

Anatomy and embryology·2006
Same journal

Nitric oxide synthase-containing neurons in the amygdaloid nuclear complex of the rat.

Anatomy and embryology·2006
Same journal

Development of cerebral sulci and gyri in fetuses of cynomolgus monkeys (Macaca fascicularis).

Anatomy and embryology·2006
Same journal

Two endothelial cell lines derived from the somite.

Anatomy and embryology·2006
See all related articles

Chick Wnt-6 gene expression was mapped throughout embryonic development. This study details Wnt-6

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • The WNT protein family comprises multiple signaling molecules crucial for embryonic development.
  • Understanding the precise roles of individual WNT members, such as Wnt-6, is essential for deciphering developmental pathways.

Purpose of the Study:

  • To isolate the full-length chick Wnt-6 cDNA.
  • To comprehensively analyze the spatiotemporal expression pattern of Wnt-6 during chick embryogenesis using in situ hybridization.

Main Methods:

  • Isolation of full-length chick Wnt-6 cDNA.
  • Whole-mount in situ hybridization to detect Wnt-6 mRNA distribution in developing chick embryos.
  • Analysis of expression patterns across various Hamburger-Hamilton (HH) stages.

Related Experiment Videos

Main Results:

  • Wnt-6 expression initiates in the ectoderm from HH-stage 4.
  • Dynamic expression patterns observed in ectoderm overlying segmental plates, epithelial somites, pharyngeal arches, and developing limbs (HH-stages 7-27).
  • Later expression restricted to feather buds and the gastrointestinal tract from HH-stage 30 onwards, with notable presence in the inner ear.

Conclusions:

  • Chick Wnt-6 exhibits a complex and dynamic expression profile throughout embryonic development.
  • The observed expression patterns suggest critical roles for Wnt-6 in ectodermal differentiation, somite development, pharyngeal arch formation, limb patterning, and organogenesis.