Related Experiment Video
Updated: Jul 12, 2026
A Tetracycline-regulated Cell Line Produces High-titer Lentiviral Vectors that Specifically Target Dendritic Cells
Published on: June 19, 2013
Generation of dendritic cells from lentiviral vector-transduced CD34+ cells from HIV+ donors
1Department of Medicine, University of California, San Diego, La Jolla, California, USA. Andreas_Gruber@dfci.harvard.edu
Abstract:
Dendritic cells hold promise as adjuvant for immunotherapy for cancer and infectious diseases. We demonstrate that a significant number of cryopreserved peripheral blood CD34(+) cells from HIV-infected subjects can be transduced with a replication-incompetent lentiviral vector expressing HIV antigens. In addition, untransduced and transduced CD34(+) cells from HIV-infected individuals were able to differentiate into dendritic cells with strong T-cell stimulatory capacity. Thus, cryopreserved CD34(+) cells from HIV-infected subjects may prove useful for immunotherapy for HIV/AIDS.
