Fluorescence enhancement from an array of subwavelength metal apertures

We report the resonant excitation of a fluorescing molecular monolayer applied to a periodic array of subwavelength apertures in a metal film. The peak fluorescence occurs under conditions of maximum transmission of the excitation light, which indicates strong enhancement of the incident light within the apertures under resonant conditions. Fluorescence output normalized to the aperture fill fraction is enhanced by nearly 40 times, and the rate of photobleaching is increased by approximately a factor of 2, suggesting an increase in the fluorescence yield.