Related Experiment Video
Updated: Feb 6, 2026

Quantification of Coenzyme A in Cells and Tissues
Published on: September 27, 2019
Protective effect of coenzyme I on mouse hematopoietic system against radiation
1Department of Oncology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. liufaquan@163.net
Objective:
To study the protective effect of NADH on the hematopoietic system of mice against radiation.
Methods:
Sixty mice were randomized into 3 groups (20/group), Group I serving as normal control group. Three days before exposure to single-dose (6.0 Gy) whole body 60Co gamma-ray irradiation in the other 2 groups, the mice in Group II received injections with 0.9% NaCl and those in Group III with intraperitoneal NADH (10 mg/kg, twice daily). The peripheral white blood cells count was carried out in the blood samples from the mouse tail vein at different time after the exposure, and the bone marrow smears were prepared with routine staining to examine the number of the granulocytes and determine their mitotic index 24 h after radiation. DNA fragmentation in mouse bone marrow cells was detected by DNA electrophoresis 24 and 48 h after irradiation.
Results:
The white blood cell count was significantly higher in mice with coenzyme I treatment before exposure to the irradiation. NADH also increased the number of the granulocytes and their mitotic index without inhibiting DNA fragmentation in the bone marrow cells as observed 24 and 48 h after the irradiation.
Conclusion:
NADH can markedly prevent the damages to the hematopoietic system in mice exposed to irradiation, but the mechanism does not involve the inhibition of bone marrow cell apoptosis.
More Related Videos
11:24Measuring DNA Damage and Repair in Mouse Splenocytes After Chronic In Vivo Exposure to Very Low Doses of Beta- and Gamma-Radiation
Published on: July 3, 2015
12:37Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
Published on: September 7, 2013
Related Concept Videos
Cofactors and Coenzymes
Cofactors and Coenzymes
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
Biological Effects of Radiation
Radiation: Applications
The average...
Absorption of Radiation
Protection of Alcohols
Protection
It defines a protecting group as the masking agent to make the more reactive species inert to a given set of conditions. This concept is depicted via the illustration of liquid flow through different outlets in an assembly of pipes. The analogy helps to understand the role...