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Updated: Sep 26, 2026
Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
Published on: June 25, 2014
Mapping and microsatellite marker development for the porcine leukemia inhibitory factor receptor (LIFR) and
A Spötter1, C Drögemüller, H Kuiper
1Institute of Animal Breeding and Genetics, School of Veterinary Medicine Hannover, Hannover, Germany. Andreas.Spoetter@tiho-hannover.de
Abstract:
Leukemia inhibitory factor receptor (LIFR), epidermal growth factor receptor (EGFR), and their respective ligands have been implicated in regulating growth and development of the early pig conceptus. We isolated a PAC clone containing the porcine gene for LIFR and a BAC clone with the porcine EGFR gene, respectively. On each of these clones one microsatellite marker was identified by sequencing a collection of subclones. These gene-associated markers were evaluated by genotyping of 202 unrelated boars of four different breeds. Based on fluorescence in situ hybridization and radiation hybrid mapping, the porcine LIFR gene was assigned to SSC16q13-->q14. The EGFR gene mapped to SSC9q26.
