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Heat shock proteins HSP27 and HSP70 in unilateral obstructed kidneys

Patricia Vallés1, Facundo Jorro, Liliana Carrizo

  • 1Department of Physiopathology, Medical School, National University of Cuyo, Centro Universitario 5500, Mendoza, Argentina. pvalles@fmed2.uncu.edu.ar

Pediatric Nephrology (Berlin, Germany)
|April 17, 2003
PubMed

Insights

Heat shock proteins HSP27 and HSP70 are upregulated in children with congenital ureteropelvic junction obstruction (UPJO). Their expression correlates with obstruction duration and impaired kidney function, suggesting an adaptive response.

Area of Science:

  • Nephrology
  • Molecular Biology
  • Pediatric Surgery

Background:

  • Unilateral ureteropelvic junction obstruction (UPJO) causes kidney injury, characterized by vasoconstriction, inflammation, oxidative stress, and fibrosis.
  • The kidney's adaptive response to congenital UPJO involves complex molecular mechanisms that are not fully understood.

Purpose of the Study:

  • To investigate the expression of heat shock proteins HSP27 and HSP70 in pediatric kidneys with UPJO.
  • To correlate HSP27 and HSP70 expression with the duration of obstruction and renal function parameters.

Main Methods:

  • Analysis of renal biopsies from 22 children with UPJO using immunohistochemical and Western blot techniques.
  • Comparison with kidney tissue from 5 control children undergoing surgery for malignancies.
  • Evaluation of kidney function, including glomerular filtration rate.

Main Results:

  • Increased expression of HSP27 was observed in proximal tubules, cortical collecting ducts, and medullary collecting ducts, correlating with obstruction duration.
  • HSP70 expression showed a similar spatial pattern to HSP27 and was linked to decreased glomerular filtration rate.
  • The constitutive form of HSP70 (HSC72) was notably absent.

Conclusions:

  • HSP27 and HSP70 play a role in the adaptive response of the human kidney to congenital UPJO.
  • These heat shock proteins may serve as biomarkers for obstruction duration and indicators of renal functional impairment.

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