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Revisiting vimentin expression in early chick development.

Christian Noack1, Felicitas Pröls, Anton J Gamel

  • 1Institute of Anatomy II, Albert-Ludwigs University Freiburg, Freiburg, Germany.

Anatomy and Embryology
|April 17, 2003
PubMed
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Researchers identified vimentin as a novel antigen in developing chick embryos using the Som5H5 antibody. This protein showed an unexpected expression pattern in the limb bud, distinct from its known roles in somitic derivatives and neural tissues.

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Immunology

Background:

  • Identifying novel antigens is crucial for understanding embryonic development.
  • Vimentin is a well-characterized intermediate filament protein with known roles in cellular structure and motility.
  • Previous studies have not detailed vimentin expression in specific embryonic domains like the proximal limb bud.

Purpose of the Study:

  • To discover somite-specific antigens with novel expression patterns in chick embryos.
  • To characterize the expression profile of the antigen recognized by the Som5H5 monoclonal antibody.
  • To investigate potential regulatory mechanisms for newly identified expression domains.

Main Methods:

  • Intrasplenic immunization of epithelial somites from developing chick embryos.

Related Experiment Videos

  • Immunohistochemical screening of various embryonic stages.
  • Western blot and mass spectrometry for antigen identification.
  • In situ hybridization using vimentin riboprobes for expression analysis.

    • Main Results:

    • The Som5H5 antibody recognized vimentin, a known protein.
    • Vimentin expression was confirmed in somitic derivatives, spinal nerves, neural tubes, and adult skeletal muscle costameres.
    • A novel vimentin expression domain was identified in the proximal region of the developing chick limb bud.
    • Signaling molecules Shh and BMP-2 did not affect this novel vimentin expression.

    Conclusions:

    • Vimentin exhibits a previously uncharacterized expression pattern in the developing chick limb bud.
    • The functional or cellular significance of this novel vimentin domain remains undetermined.
    • Further research is needed to elucidate the role of vimentin in this specific developmental context.