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Measurement of Factor V Activity in Human Plasma Using a Microplate Coagulation Assay
Published on: September 9, 2012
[Isolation, purification, and properties of factor XII and its active fragment (beta-XIIa)]
T B Blokhina1, E A Neshkova, G A Iarovaia
1Russian Medical Academy of Postgraduate Education, Barrikadnaya st., 2, Moscow, 123995 Russia.
Voprosy Meditsinskoi Khimii
|April 18, 2003
Summary
A new, faster method isolates human blood plasma prekallikrein, coagulation factor XII, and beta-XIIa. This technique yields homogeneous preparations suitable for preparative quantities, improving efficiency in factor isolation.
Area of Science:
- Biochemistry
- Hematology
Context:
- Human blood plasma contains crucial proteins involved in coagulation and inflammation.
- Efficient isolation of these proteins is essential for research and therapeutic applications.
Purpose:
- To develop an improved procedure for isolating human plasma prekallikrein, coagulation factor XII, and its active fragment beta-XIIa.
- To achieve high purity and specific activity of the isolated factors.
Summary:
- A novel isolation procedure combining traditional chromatography and Fast Protein Liquid Chromatography (FPLC) was established.
- Disc-electrophoresis confirmed the homogeneity of isolated factor XII and beta-XIIa.
- The method is less time-consuming and enables isolation in preparative quantities, with specific activities of 70.6 U for factor XII and 2.5 U for beta-XIIa.
Impact:
- Provides a more efficient method for obtaining key coagulation factors.
- Facilitates further research into the roles of prekallikrein, factor XII, and beta-XIIa in hemostasis and related pathways.
- Enables larger-scale production for potential clinical or research use.
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